Hoʻohana ʻia ka kit no ka in vitro qualitative ka ʻike ʻana i nā genes ORF1ab/N/S o ka novel coronavirus 2019-nCoV i nā specimens respiratory me nā swabs oropharyngeal, nasopharyngeal swab, sputum a me ka hana ʻana i ka mutation type ma HV69- 70 del i ka manawa like.E like me ka hōʻike ʻana ma ka waihona GISAID, ʻoi aku ma mua o 95% Omicron nā ʻano ʻano ʻano like ʻole i hōʻike ʻia me ka holoi ʻana i ka HV69-70, hiki ke kumu i kahi S gene target failure (SGTF) i nā hoʻokolohua PCR.Hiki ke hoʻohana ʻia ʻo SGTF ma ke ʻano he māka koho no ka pale ʻana no Omicron.
Hoʻolālā ʻia nā pūʻulu kumu mua a me FAM labeled probe no ka ʻike kikoʻī o ka ORF lab gene of 2019-nCoV, VIC labeled probe no N gene of 2019-nCoV, ROX labeled probe no S gene HV69-70 del mutation o 2019-nCoV.Hāʻawi ke kanaka RNase P gene i ka manawa like me ka hōʻike hoʻāʻo e hāʻawi i kahi mana kūloko e hōʻoia i ke kaʻina hana nucleic extraction a me ka pono reagent.Hoʻopaʻa inoa ʻia ʻo Probe e huli ana i ke kanaka RNase P gene me CY5.
Pūnaehana PCR manawa maoli: Aehealth Diagenex AL, ABI 7500, ViiATM 7, Quant Studio 7 flex.Roche Lightcycler 480, Agilent Mx3000P/3005P, Rotor-GeneTM6000/0.Bio-Rad CEX96 Touch TM SLAN-96S.SLAN-96P, MA 6000;