Hoʻohana ʻia kēia pahu no ka ʻike in vitro qualitative nucleic acidMonkeypox Virus i loko o ke kanaka serum, lesion exudate samples anā hōʻailona scab.Hoʻolālā ʻia nā pūʻulu kumu mua a me FAM labeled probesno ka ʻike kikoʻī o ka Monkeypox Virus, Human RNase P geneunuhi like me ka hāpana ho'āʻo hāʻawi i lokomana e hōʻoia i ke kaʻina hana nucleic extraction a me reagentpono.Hoʻopaʻa inoa ʻia ka probe e huli ana i ke kanaka RNase P gene me VIC.
| Nā ʻāpana | 48 Nā hoʻāʻo/kit | 96 Nā hoʻāʻo/kit |
| ʻO ka pale ʻana o ka PCR | 672 μL × 1 paipu | 672 μL × 2 paipu |
| ʻO ka hui ʻana o ka enzyme PCR | 50 μL × 1 paipu | 100 μL × 1 paipu |
| Manaʻo maikaʻi | 100 μL × 1 paipu | 200 μL × 1 paipu |
| Manaʻo ʻino | 100 μL × 1 paipu | 200 μL × 1 paipu |
1. ʻIke: 200 kope / mL.
2. Mea kiko'ī: ʻAʻohe hopena keʻa me Enterovirus(EV), Measlesma'i (MV), ma'i ma'i Rubella (RV), ma'i virus Varicella-zoster (VZV),Virus Dengue(DenV), KanakaParvovirus B19(HPVB19),ʻO ka maʻi maʻi Epstein-barr (EBv), ka maʻi herpes kanaka 6(HHV-6)
3. Pono: CV ≤ 5%.
Pūnaehana PCR manawa maoli: Diagenex AL, ABI 7500, ViiATM 7, QuantStudio 5, QuantStudio 6/7 pro, QuantStudio 6/7 flex, Agilent Mx3000P/3005P, Rotor-GeneTM 6000/Q, Bio-Rad/CFX96 Touch ，Hongshi SLAN-96S/96P，AGS8830, AGS4800