| 64T | 96T | ||
| ʻāpana | ʻAiʻai | ʻāpana | ʻAiʻai |
| Pāpā hoʻopaʻa | 4 | Lysis pale B | 2 |
| Lysis pale B | 1 | Papa Lysis | 1 |
| lima ʻili | 8 | Holoi 1 pā | 1 |
| Papa kuhikuhi | 1 | Holoi 2 papa | 1 |
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| Pāʻani elution | 1 |
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| lima ʻili | 1 |
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| Papa kuhikuhi | 1 |
96-well Poepoe Papa Hoomakaukau
Nā ʻāpana 64T i ka pā punawai e like me kēia:
| Kahua-Wei | 10r7 | 2or8 | 3 a i ʻole 9 | 4or10 | 5orll | 6orl2 |
| Kit ʻāpana | ʻO Lysis Hoʻopaʻa 600μL | Holoi Hoʻopaʻa1 500μL | Holoi Hoʻopaʻa2 500μL | Pākaʻa | Magnetic Nā lei 310μL | Elute Hoʻopaʻa l00μL |
Fa i ʻole 64T kime:
E wehe pono i ke kiʻi hoʻopaʻa wela ma ka pā reagent, a laila hoʻohui i 200μL o ka laʻana a me 20μL o lysis buffer B i loko o ke kolamu 1/7 o ka pā reagent.
No ka pahu 96T:
E wehe pono i ke kiʻi hoʻopaʻa wela ma ka pā reagent, a laila hoʻohui i 200μL o ka laʻana a me 20μL o lysis buffer B i loko o ka pā lysis.
E hoʻokomo i ka pā reagent a me ka lima plastic i loko o ke kūlana i koho ʻia o ka mea kani ma ke ʻano, a laila kaomi e holo i ka papahana "DNA/RNA" unuhi ma ka nucleic acid extractor.
I ka pau ʻana o ka papahana, e wehe i ka lima lima a hoʻolei.
Wehe i ka pā elution, a lawe ʻia ka eluent a mālama ʻia i loko o kahi paipu centrifuge hou no nā hoʻokolohua ma lalo.Inā ʻaʻole hiki ke hoʻokō ʻia ka hoʻokolohua ma lalo i ka manawa, hiki ke mālama ʻia ka laʻana DNA ma -20 ℃ a hiki ke mālama ʻia ka laʻana RNA ma -80 ℃.