Hoʻopili ka Spike glycoprotein receptor-binding domain (RBD) o SARS-CoV-2 i ka hoʻopaʻa ʻana o ka mea maʻi viral i ka angiotensin-converting enzyme 2 (ACE2) receptor ma ka ʻili o nā cell kanaka.No laila, ʻo ka launa pū ʻana o Spike-ACE2 he mea hoʻoholo koʻikoʻi no ka maʻi maʻi maʻi.
Wahi a ka noiʻi hou mai ka 'Oihana Ola o UK, e pili ana i ka ʻikepili o ka genome sequencing holoʻokoʻa, epidemiology a me ka hoʻohālikelike ʻana, ʻo ka maʻi o nā ʻano like ʻole o SARS-CoV-2 ua aneane 40-70% kiʻekiʻe ma mua o nā mea ʻepekema hoʻololi ʻē aʻe i ʻike ʻia. .ʻO ka pane wikiwiki a me ka ʻike ʻana kekahi o nā mea koʻikoʻi o ka pale a me ke ana hoʻomalu.
Hoʻohana ʻia ka pahu no ka in vitro qualitative ka ʻike ʻana i nā genes ORF1ab/N/S o ka novel coronavirus 2019-nCoV i loko o nā specimens hanu, a me ka hana ʻana i ka mutation type ma N501Y i ka manawa like.
Hoʻohana ka kit i ke ʻano ARMS, lawe i ka S gene o ka novel coronavirus (2019-nCoV) ma ke ʻano he wahi i hoʻopaʻa ʻia no ke kākau ʻana i ka mutation, hoʻolālā i nā primers kikoʻī a me nā noiʻi no N501Y.
| Nā huahana | Nā kikoʻī | Hale kūʻai temp | wehewehe |
| ʻO ka pahu PCR manawa maoli no ka NovelʻO Coronavirus 2019-nCoV aʻO ka Novel Coronavirus 2019-nCoVHoʻololi Gene (N501Y) | 48T | -20 ℃ | Analytes: Wild-type o N501Y,ʻAno mutant o N501Y, ORF1ab,N gene a me ka hooponopono Kuloko |
Ka hoʻolike like ʻana o ka Template
- Oropharyngeal Swabs
- Nasopharyngeal Swab
- ʻO Bronchoalveolar Lavage Fluid
- Sputum
Nā ʻāpana pahu
- RT-PCR ho'opa'a ho'opa'a
- ʻO ka hui ʻana o ka enzyme RT-PCR
- Mana maikaʻi ʻano ʻāhiu
- Hoʻololi ʻia ka mana maikaʻi
- Manaʻo ʻino
N501Y ʻano hoʻololi
