Aia nā coronaviruses hou i ka genus B.ʻO COVID-19 kahi maʻi maʻi ʻeha hanu.Hiki ke maʻalahi nā kānaka.I kēia manawa, ʻo nā maʻi i loaʻa i ka coronavirus novel ke kumu nui o ka maʻi;Hiki i nā kānaka maʻi asymptomatic ke lilo i kumu maʻi.Ma muli o ka noiʻi epidemiological o kēia manawa, ʻo ka manawa incubation he 1 a 14 mau lā, ʻo ka hapa nui he 3 a 7 mau lā.ʻO nā hōʻike nui e pili ana i ke kuni, ka luhi a me ka ʻū maloʻo.Loaʻa ka nasal congestion, runny ihu, ʻeha ʻāʻī, myalgia a me ka maʻi maʻi i kekahi mau hihia.
Hoʻohana ʻia kēia pahu no ka ʻike in vitro qualitative o ka novel coronavirus 2019-nCov i nā ʻano hanu e pili ana i ka swab oropharyngeal, sputum, bronchoalveolar lavage fluid a me nasopharyngeal swab.Hoʻolālā ʻia nā pūʻulu kumu mua a me FAM labeled probe no ka ʻike kikoʻī o ka gene ORFlab o 2019-nCov.ʻO ka noiʻi hōʻailona VIC no N gene o 2019-nCov.Hāʻawi ke kanaka RNase P gene i ka manawa like me ka hōʻike hoʻāʻo e hāʻawi i kahi mana kūloko e hōʻoia i ke kaʻina hana nucleic extraction a me ka pono reagent.Hoʻopaʻa inoa ʻia ʻo Probe e huli ana i ke kanaka RNase P gene me CY5.
| Nā ʻāpana | 50 ho'āʻo / pahu |
| RT-PCR Reaction Mix Reagent | 240μL × 1 paipu |
| Mea hoʻohuihui Enzyme | 72μL ×1 paipu |
| ʻO 2019-nCoV ka hoʻokolokolo mua | 48μL ×1 paipu |
| Manaʻo maikaʻi | 200μL × 1 paipu |
| Manaʻo ʻino | 200μL × 1 paipu |
ʻIke: 200 kope/mL.
Mea kiko'ī: ʻAʻohe hopena kea me SARS-CoV, MERS-CoV, CoV-HKU1, CoV-OC43, CoV-229E, CoV-NL63 a me HIN1, H3N2, H5N1, H7N9, Influenza B, Parainfluenza Virus (123), Rhinovirus(A ,B,C), Adenovirus (1,2,3,4,5,7,55), Human interstitial pneumovirus, Human metapneumovirus, EBv, Measles virus, Human cytomegalic virus, Rota virus, Norovirus, Mumps virus, Varicella Zoster Virus , Mycoplasma pneumonia, Chlamydia pneumonia, Legionella, Bordetella pertussis, Haemophilus influenza, Staplhylococcus Aureus, Streptococcus Pneumonia, Streptococcus pyogenes, Klebsiella pneumonia, Tuberculous bacillus, Aspergillus fumigatus, Candida albicans, Cryptococcus glabrataform.
Ka pololei: CV ≤5%.
Pūnaehana PCR manawa maoli: Aehealth Diagenex AL, ABI 7500, ViiATM7, Quant Studio 7 flex.Roche Lightcycler 480, Agilent Mx3000P/3005P, Rotor-GeneTM6000/0.Bio-Rad CEX96 PaʻiTMSLAN-96S.SLAN-96P