Hoʻohana ʻia kēia pahu no ka in vitro qualitative nucleic acid detection of Monkeypox Virus a me ke kikowaena / komohana ʻApelika clade i ka Human serum, lesion exudate samples a me scab specimens.Hoʻolālā ʻia nā pūʻulu kumu mua a me FAM labeled probe no ka ʻike kikoʻī ʻana i ka Monkeypox Virus, ROX labeled probe no ka waena o ʻApelika clade, CY5 labeled probe no ka ʻĀpelika komohana.Hāʻawi ke kanaka RNase P gene i ka manawa like me ka hōʻike hoʻāʻo e hāʻawi i kahi mana kūloko e hōʻoia i ke kaʻina hana nucleic extraction a me ka pono reagent.Hoʻopaʻa inoa ʻia ka probe e huli ana i ke kanaka RNase P gene me VIC.
| Nā ʻāpana | 48 Nā hoʻāʻo/kit | 96 Nā hoʻāʻo/kit |
| ʻO ka pale ʻana o ka PCR | 672 μL × 1 paipu | 672 μL × 2 paipu |
| ʻO ka hui ʻana o ka enzyme PCR | 50 μL × 1 paipu | 100 μL × 1 paipu |
| Manaʻo maikaʻi | 100 μL × 1 paipu | 200 μL × 1 paipu |
| Manaʻo ʻino | 100 μL × 1 paipu | 200 μL × 1 paipu |
1. ʻIke: 200 kope / mL.
2. Mea kiko'ī: ʻAʻohe hopena keʻa me Enterovirus(EV), Measles virus (MV), Rubella virus (RV), Varicella-zoster virus (VZV), Dengue virus (DenV), Human Parvovirus B19 (HPVB19), Epstein-barr virus (EBv), maʻi herpes kanaka 6(HHV-6)
3. Pono: CV ≤ 5%.
Pūnaehana PCR manawa maoli: Diagenex AL, ABI 7500, ViiATM 7, QuantStudio 5, QuantStudio 6/7 pro, QuantStudio 6/7 flex, Agilent Mx3000P/3005P, Rotor-GeneTM 6000/Q, Bio-Rad/CFX96 Touch ，Hongshi SLAN -96S/96P，AGS8830, AGS4800